DNA Isothermal Amplification Kit NFO with 500-1000 Copies/UL Detection Limit 48 Tests / Box

Product DescriptionAmp-future DNA Isothermal Amplification Kit NFO with 500-1000copies/µL Detection Limit, 48 Tests/box, -20°C StorageProduct DetailKit Storage and term of ValidityStorage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.Term of Validity: 14 months Isothermal nucleic acid Principle SummaryThe kit is based on room and constant temperature nucleic acid rapid amplification technology, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3' end of the primer to start the chain extension. The kit relies on the role of NFO enzyme and adds the designed specific molecular probes according to the template, and get the result by colloidal gold technology (sandwich method). Technical Parameters:ParametersDetailsProduct NameDNA Isothermal Amplification Kit NFOManufacturerAmp-futureStorage Temperature-20°CKit ComponentsEnzymes, Buffers ,ReagentsPackaging48 Tests/boxDetection Limit500-1000copies/µLShippingICETest Time5-20mins Isothermal nucleic acid Product Features1/ High sensitivity and specificity, short reaction time.2/ The reagent form is freeze-dried, stable and easy to operate.3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus. Isothermal nucleic acid ApplicationsSuitable for DNA isothermal rapid amplification kit(NFO type)Primer: Require pair of nucleotide primers with the length of 25-35 bp.DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes. Notes1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.